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KMID : 1007520150240051837
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Food Science and Biotechnology
2015 Volume.24 No. 5 p.1837 ~ p.1843
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The Inhibitory Effects of Citrus unshiu Markovich Extracts on the Receptor Activator of Nuclear Factor-Kappa-B Ligand-Mediated Osteoclast Differentiation and Function
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Baek Jong-Min
Min Jung-Youl
Kim Ju-Young
Yoon Kwon-Ha
Choi Min-Kyu
Oh Jae-Min
Lee Myeung-Su
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Abstract
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Ingestion of Citrus unshiu Markovich (CUM) has been widely used as an alternative therapy for thousands years in Korean traditional medicine. Both anti-osteoclastic actions of CUM extracts (CUME) and the possibility for development as a healthy food product were investigated. CUME significantly (p<0.05) and dose-dependently diminished the receptor activator of nuclear factor kappa- B ligand (RANKL)-induced formation of tartrate resistant acid phosphatase (TRAP)-positive osteoclasts. At the molecular level, CUME interrupted osteoclastogenesis based on selective stimulation of ERK and Akt activation. CUME caused down-regulation of osteoclast-specific marker gene expression derived from impairment of c-Fos and nuclear factor of activated T cells c1 (NFATc1) activation. Treatment with CUME caused localization failure of filamentous actin (F-actin) rings during cellularization and, subsequently, mature osteoclasts suffered malfunction of osteolytic activity. CUME has potential for prevention of osteoporosis.
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KEYWORD
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Citrus unshiu Markovich, osteoclast, bone resorption, osteoporosis
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